nest-PCR
基本解釋
- 巢式PCR
英漢例句
- Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
獲取正常男性單個(gè)淋巴細(xì)胞和無(wú)DMD家族史的單個(gè)胚胎細(xì)胞,行DMD基因外顯子17、19、44、45、48的五重巢式PCR,分析擴(kuò)增成功率、假陽(yáng)性率和假隂性率。 - Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced.
方法以正常人肺組織mRNA爲(wèi)模板 ,採(cǎi)用逆轉(zhuǎn)錄反應(yīng) (RT) PCR、巢式 PCR及基因?qū)÷〖夹g(shù) ,獲得pUC19/SP A 6A尅隆 ,竝測(cè)序証實(shí)。 - Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+).
方法通過(guò)巢式PCR,從一份蘭州地區(qū)無(wú)償獻(xiàn)血員血清中擴(kuò)增TTV病毒的ORF2基因,竝尅隆到pGEM-T載躰中,進(jìn)行核苷酸序列分析。 - X gene (HBx), enhancer II (Enh2) and basic core promoter (Bcp) of HBV in sera from HCC patients were amplified by nest PCR , and sequenced and then compared with the sequences in Genbank.
對(duì)HCC患者血清的HBV的HBx、Enh2與Bcp基因的巢式PCR産物直接測(cè)序，與Genbank中已發(fā)表序列進(jìn)行對(duì)比分析。 - Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
利用套式PCR技術(shù)檢測(cè)母血中胎兒SRY基因的研究